Unus Gradus RT-qPCR probe ornamentum
Descriptio
Unus Gradus qRT-PCR Probe Ornamentum specialiter destinatur pro qPCR qui directe utitur RNA (eg virus RNA) ut template.Utens gene specialibus primariis (GSP), inversa transcriptio et qPCR finiri possunt in uno tubo, signanter minuendo pipetting processuum et periculum contaminationis.inactivum esse potest in 55° sine afficiens efficientiam et sensibilitatem ipsius qRT-PCR.Unus Gradus qRT-PCR Probe Ornamentum praebetur in Magistro Mix.The 5 Unus Gradus Mix continet quiddam optimized et dNTP/dUTP Mix, et idoneum ad systema deprehensionis summae speciei innixum in fluorescentibus proborum intitulatis (eg TaqMan).
Principia fundamentalia RT-qPCR
specificatio
| Test Items | Specification | Proventus |
| (SDS PAGE) Puritas Enzyme Stock (SDS PAGE) | ≥95% | Transi |
| Endonuclease Actio | Non detectum | Transi |
| Exodlease Actio | Non detectum | Transi |
| Rnase Actio | Non detectum | Transi |
| Residua E.coli DNA | ＜1copies/60 | Transi |
| Eget Assay-Ratio | XC% ≤110% | Transi |
Components
| Components | 100rxns | 1,000rns | 5,000 rxns |
| RNAse-gratis ddH2O | 2*1ml | 20ml | 100ml |
| V * gradum miscere | 600μl | 6*1ml | 30ml |
| Unum gradum enzyme misce | 150μl | 2* 750μl | 7.5ml |
| 50* ROX reference Dye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX reference Dye 2 | 60μl | 600μl | 3*1ml |
a.One-Step Buffer includit dNTP Mix et Mg2+.
b.Enzyme Domine maxime continet contra
transcriptase, Hot Committitur Taq DNA polymerase (anticorporis modificatio) et Rnase inhibitor.
c.Usus est ad corrigendum errorem sinalium fluorescens inter puteos diferentium.
d.ROX: Calibrationem eligere debes secundum exemplar instrumenti probati.
Applications
QPCR deprehensio
Shipping and Storage
Naviportans:Ice sarcinas
Conditiones repono:Copia apud -20℃.
Vita Shief:18months
