Ultra Nuclease
UltraNuclease est machinatoredendonuclaesus genetice ex Serratia marcescens derivata, quae DNA vel RNA degradare potest, vel duplici vel simplici subducto, lineari vel circulari sub amplis conditionis, acida nucleica in 5'-monophosphata oligonucleotides cum 3-5 basi longitudinis omnino degradatur. .Post modificationem machinalem geneticam, productio fermentata, expressa et purificata in Escherichia coli (E. coli), quae inscientificam inquisitionem lysatae cellae supernatantis et cellulae lysatae minuit, sed etiam auget purificationem efficientiam et inquisitionem functionis ofprotein.Potest etiam adhiberi in therapia gene, virus purificationis, productionis vaccini, interdum et polysaccharidis pharmaceuticae industriae sicut hospes residua acidi nuclei remotionis gerentis.
Product Features
| CAS Non. | 9025-65-4 |
| EC No. | |
| M. Pondus | 30kDa |
| Isoelectric Point | 6.85 |
| Dapibus Puritas | ≥99%（ SDS-PAGE & SEC-HPLC） |
| Imprimis Actio | ≥1.1×106U/mg |
| OptimumTemperature | 37°C |
| optimum pH | 8.0 |
| ProteaseActivity | negans |
| Bioburden | 10CFU/100,000U |
| Residua Host-cellae dapibus | ≤10ppm |
| Grave metallum | ≤10ppm |
| Bacterial Endotoxin | 0.25EU/1000U |
| Repono Buffer | 20mM Tris-HCl, pH 8.0, 2mM MgCl2 20mM NaCl 50% Glycerolum |
Repono conditionibus
≤0°C translation-25~-15°C Repono，2 annorum validitas (devitanda congelatio tabida).
Unitas Definition
Moles enzyme ad absorptionem valoris △A260 ab 1.0 intra 30 min ad 37 °C, pH 8.0 mutandam adhibita, aequivalens sperma salmonum 37μg DNA digesto in oligonucleotidas secando, unitas activa definita est (U).
Regimen quālitātis
Residua Host-cellae dapibus: ELISA ornamentum
•Protease Residua: 250KU/mL UltraNuclease portavit cum substrata pro 60min, nulla detecta est actio.
•Bacterial Endotoxin: LAL-Test, Pharmacopoea Reipublicae Popularis Sinarum Libri IV (2020 Edition) Modus test methodi Gel.Regulae generales (1143).
•Bioburden: Pharmacopoeia Reipublicae Popularis Sinensis Volume 4 (2020 Edition) — General
Rules for Sterility Test (1101), PRC National Standard, GB 4789.2-2016.
•Grave metallum:ICP-AES, HJ776-2015.
Operatio
UltraNuclease actio signanter inhibuit cum SDS intentio super 0.1% vel EDTA
retrahitur per 1mM.Surfactant Triton X- 100, Inter 20 et Twende 80 nullum effectum in nucleasi habuit.
possessiones cum retrahitur sub 1.5%.
| Operatio | Operatio meliorem | Operatio valida |
| Temperature | 37℃ | 0-45℃ |
| pH | 8.0-9.2 | 6.0- 11.0 |
| Mg2+ | 1-2mM | 1- 15mM |
| DTT | 0- 100mM | >100mM |
| 2-Mercaptoethanol | 0- 100mM | >100mM |
| Metallum ion -Na+, K+ etc. | 0-20mM | 0-200mM |
| PO43- | 0- 10mM | 0- 100mM |
Usus et dosage
• Exogenosum acidum nucleicum ex vaccino producto remove, periculum residua acidi nuclei toxicitatis minuere et productum saluti emendare.
• Viscositatem cibi liquoris ex acido nucleico minuendo, processui temporis minuendi et dapibus cede augendi.
• Aufer acidi nuclei quod involvit particula (virus, inclusio corporis, etc.), quod conducit
ad remissionem et purificationem particulae.
| Type Experimentalis | Dapibus Productio | Vitus, Vaccine | Cellula Medicamenta |
| Cellulae Number | 1g infectum pondus cellula (Resuspended quiddam cum 10ml) | 1L fermentationem liquid supernatant | 1L cultura |
| Minimum Dosis | 250U | 100U | 100U |
| Commendatur Dosis | 2500U | 25000U | 5000U |
• Curatio nuclei meliorem solutionem et receptam specimen pro columnae chromatographia, electrophoresi et analysi deletione emendare potest.
• In therapia gene, acidum nucleicum ad obtinendum virus adeno-associatum depuratum tollitur.
