Bst 2.0 DNA Polymerase (Glycerolum liberum, alta densitas)
Bst DNA polymerasis V2 derivatur ab Bacillo stearothermophilo DNA Polymerase I, quod actio polymerasis 5′ →3′ et catena valida postea activitatem exonucleasem habet, sed non est 5′ →3′ exonucleasis actio.Bst DNA Polymerase V2 optime convenit ad locum subductionis, amplificationis isothermal LAMPIS (Loop amplificationis isothermal mediatae) et celeris sequelae.Haec Bst DNA polymerasis V2 potest inhibere DNA polymerasi activitatem in cella temperie, ita ut exerceri possit et ratio reactionis formari potest ad temperaturas locus, impediendo non specificas amplificationes et amplificationem efficientiam reactionis, et haec versio potest. lyophilized potest.Praeterea capax est suam actionem in calidis temperaturis exsolvere, ita necessitatem separati activitatis gradus eliminare.
Components
| Component | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (Glycerolum-free) (32U/μL) | 0.05 mL | 0.25 mL | 2.5 mL |
| 10×HC Bst V2 Buffer | 1.5 mL | 2×1.5 mL | 3×10 mL |
| MgSO4 (100mM) | 1.5 mL | 2×1.5 mL | 2×10 mL |
Applications
1.LUMEN isothermal amplificatio
2.DNA litus unum obsessio reactionem
3.Princeps GC gene sequencing
4.DNA sequencia nanogram massa.
Repono Condition
Transportatio sub 0°C et reponenda -25°C~-15°C.
Unitas Definition
Una unitas definitur quantum enzyme, quod 25 nmol de dNTP in acidum insolubilem materiae 30 minutarum 65°C incorporet.
LUMEN reactionem
| Components | 25 μLSystema |
| 10×HC Bst V2 Buffer | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTPs (10mM quisque) | 3.5 μL |
| SYTO™ 16 Green (25×)a | 1.0 μL |
| Primario mixb | 6 μL |
| Bst DNA Polymerase V2 (Glycerolum-free) (32 U/uL) | 0.25 μL |
| Formula | μL |
| ddH₂O | Usque ad 25 μL |
Notae:
1) a.SYTOTM 16 Sedum (25×): Iuxta necessitates experimentales aliae colores in substitutis adhiberi possunt;
2) b.Prima mix: permixtione 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB et alia uolumina.
Reactio et Condition
1 HC Bst V2 Buffer, temperatura incubationis est inter 60°C et 65°C.
Calor Inactivation
80°C, 20 min.
