RT-LAMP Fluorescent (globus Lyophilized)
RT-LAMP Fluorescent Magister Mix (Powder Lyophilised) quiddam continet reactionem, Bst DNA polymerases et calor repugnant transcriptase, lyophilised tutelaris et tinctura fluorescentium.Productum in globuli forma lyophilized, et solum primariis et exemplaribus ad usum addi oportet.
Reagens Component
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Consuetudinem
Nam DNA vel RNA isothermal amplificatio
Repono Conditions
Transporto et horrea ad 2~8°C.
Shelf life: 12 months.
Protocol
1. Sume numerum respondentem globuli lyophilized secundum numerum testium.
2. Reactio ratio praeparationis
| Component | Dosis |
| RT-LAMP Fluorescent Master Mix (Pulvis Lyophilized) | 2 globuli |
| Tinctura fluorescent | x μL |
| 10 Primer Mix a | 5 μL |
| Templates DNA/RNA b | n μL |
| Totalis | 50 μL |
Notae:
1) a.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) b.Exemplum acidum nuclei commendatur in aqua DEPC dissolvenda.
Hyasen Biotechnology Co., Ltd
III) reactionem progressio
| Temperature | Tempus | Cycles |
| 65 | 60s a | 30 |
Notae:
1. Oratio fluorescent signum
Notae
1. Motus temperatus potest optimized inter 62°C et 68°C secundum primariumConditiones;
2. Experimentum normae operationis, inclusa praeparatione systematis reactionis, considerare debet;sample process and dosing;
3. Ad contaminationem vitandam, suadetur ut systema reactionem in mensa ultra-mundana praeparare possitet exempla adde fumi cucullam in aliis conclavibus ad vitanda falsa impedimenta positiva.
