D-Lactate dehydrogenase(LDH)

Cas No

EC No.:1.1.1.27

Sarcina: 10ku, 100ku, 500ku, 1000ku

Product Detail

Descriptio

Enzyme adhibetur ad remotionem pyruvatis in determinationibus laborantis cum NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenas).

Consuetudinem

1. Alkaline phosphatasi cum servo (antibodiorum, streptavidin etc.) specifice clypeum molecularum cognoscere potest, ac adhiberi potest in ELISA, WB et detectioni histochemicae;
2. Alkaline phosphatase adhiberi potest ad dephosphorizandum 5 '-terminalem DNA vel RNA ad impediendam sui conjunctionem;
3. Supra dephosphorylated DNA vel RNA per phosphates radiophonicos intitulatum potest (per T4 poly-nucleotide kinase)

Chemical Structure

Reactionem mechanism

D-Lactate + NAD⁺→ Pyruvate +NADH + H⁺

Specification

Test Items	Specifications
Descriptio	Pulvis albus amorpho, lyophilized
Actio	≥200U/mg
Puritas (SDS-PAGE)	≥90%
Solubilitas (10mg pulveris / mL)	Patet
NADH/NADPH oxidase	≤0.01%
Malate dehydrogenase	≤0.005%
Glutamate dehydrogenase	≤0.003%
Pyruvate kinase	≤0.03%
Dehydrogenase glutamate	≤0.003%
Aspartate aminotransferase	≤0.001%
Alamine aminotransferase	≤0.001%