Phosphatase Alkaline (ALP)

Cas No

EC No.:3.1.3.1

Sarcina: 100μL, 500μL, 10mL, 100mL, 1000mL

depictio producti

Descriptio

Phosphatase alcalina derivatur a recombinante E. coli cola qui gena TAB5 portat.Enzyme catalysma dephosphorylationem 5´ et 3´ fines DNA et RNA phosphomonoestrorum exstinguit.Tum hydrolyses ribosum, deoxyribonucleosidum ac triphosphates (NTPs et dNTPs).TAB5 Phosphatase Alkaline agit in 5° prominentibus, 5´ reductis et obtusis finibus.Phosphatase in multis applicationibus biologiae hypotheticae adhiberi potest, ut delineatio vel exploratio finis labellae ad phosphorylatos fines DNA vel RNA removendos.Dephosphorylatio in experimentis exquisitis impedit DNA plasmida linearizata a auto-ligatione.Potest etiam dNTPs in pcr reactiones unincorporatas degradare ut exemplum praeparet DNA sequencendi.Enzyme omnino et irrevocabiliter inactivated calefaciendo ad 70°C pro 5 minutis, ita ut remotionem phosphatasis ante ligationem vel finem labelandi superfluum.

Consuetudinem

1. Alkalina phosphatasi cum servo (antibodiorum, streptavidin etc.) specifice clypeum molecularum cognoscere potest, ac adhiberi potest in ELISA, WB et detectioni histochemicae;
2. Alkaline phosphatase adhiberi potest ad dephosphorizandum 5 '-terminalem DNA vel RNA ad impediendam sui conjunctionem;
3. Supra dephosphorylated DNA vel RNA per phosphates radiophonicos intitulatum potest (per T4 poly-nucleotide kinase)

Chemical Structure

Specification

Test Items	Specifications
Enzyme Actio	5U/μL
Endonuclease Actio	Non detectum
Exonuuclease Actio	Non detectum
Nicking Activity	Non detectum
RNase Actio	Non detectum
E.coli DNA	≤1copy/5U
Endotoxin	LAL-Test, ≤10EU/mg
Puritas	≥95%