Ribonuclease A (RNase A)

Ribonuclease A (RNase A) est polypeptidis unico subducta continens vincula 4 disulfit cum pondere hypothetico circiter 13.7 kDa.RNase A est endoribonuclease quae speciatim RNA destitutum in C et U residua deducit.Speciatim synthesis cognoscit vinculum phosphodiestri formatum per 5'-ribosam globi nucleotidis et phosphatae in 3'-riboso pyrimidine nucleotide adiacenti, ita ut 2', 3' - phosphates cyclici hydrolytici respondentibus. 3'-phosphates nucleosides (eg, pG-pG-pC-pA-pG adglutinatur RNase A ad generandum pG-pG-pCp et A-PG).RNase A acerrimus est in RNA inhaerendo unico destituto et in variis reactionis condicionibus operatur: in humilibus concentrionibus salsis (0 ad 100 mM NaCl), adhiberi potest ut RNA destitutis, subductis RNA et RNA fila in RNA-DNA hybrids.dum in concentratione alto salis (≥0.3 M), RNase A specialiter adhaerere potest RNA subductis.