T4 RNA ligase
Descriptio
Productum hoc est ligamentum ATP-dependens T4 RNA (T4 RNA Ligase I) recombinante expressum ab E. coli.Hoc enzyme catalyses formationis vinculorum phosphodiestri inter oligonucleotides, subductis RNA et DNA intermolecular/ intra-molecular 5'-PO4 et 3'-OH.
Unitas Definition
Una unitas definitur quantum enzyme requiritur ad 1 nanomolum convertendi 5´-[³²P]rA16 in formam phosphatasim resistentem in 30 minutis ad 37°C.
Specification
| Test Items | Specifications |
| Enzyme Actio | 10 U/μL |
| Dapibus Actio | ≥ 95% |
| Nickase | Non detectable |
| RNase Actio | Non detectable |
| Ecoli gDNA | ≤1copy/50U |
| Endotoxin | LCL-Test, ≤10EU/mg |
Transportatio et repono
Transportatio:Gelu siccum
Repono:Copia ad -25 ~ -15 ° F (devitare saepe frigore tabidaque)
Commendatur re- test Vita:2 year
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