Alkaline Phosphatase(ALP)
Descriptio
Phosphatase alcalina derivatur a recombinante E. coli cola qui gena TAB5 portat.Enzyme catalysma dephosphorylationem 5´ et 3´ fines DNA et RNA phosphomonoestrorum exstinguit.Item hydrolyses ribosas, et deoxyribonucleosides triphosphates (NTPs et dNTPs).TAB5 Phosphatase Alkaline agit in 5° prominentibus, 5´ reductis et obtusis finibus.Phosphatase in multis applicationibus biologiae hypotheticae adhiberi potest, ut delineatio vel exploratio finis labellae ad phosphorylatos fines DNA vel RNA removendos.Dephosphorylatio in experimentis exquisitis impedit DNA plasmida linearizata a auto-ligatione.Potest etiam dNTPs in pcr reactiones unincorporatas degradare ut exemplum praeparet DNA sequendi.Enzyme totaliter et irrevocabiliter incavetur calefaciendo ad 70°C per 5 minuta, inde removendo phosphatasim ante ligationem vel finem labellae superfluae.
Chemical Structure
Specification
| Test Items | Specifications |
| Enzyme Actio | 5U/μL |
| Puritas | ≥ 95% |
| Endonuclease Actio | Non detectable |
| Exonuclease Actio | Non detectable |
| Nicking Activity | Non detectable |
| RNase Actio | Non detectable |
| E.coli DNA | ≤1copy/5U |
| Endotoxin | LAL-Test, ≤ 10EU/mg |
Transportatio et repono
Transportatio:Ice sarcinas
Repono:Copia ad -25 ~ -15 ° F (devitare saepe frigore tabidaque)
Commendatur re- test Vita:2 year
